VEGF and PlGF promote adult vasculogenesis

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VEGF and PlGF promote adult vasculogenesis

Postby patoco » Fri Sep 22, 2006 10:31 am

VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization.

June 2006

Li B, Sharpe EE, Maupin AB, Teleron AA, Pyle AL, Carmeliet P, Young PP.

Departments of *Pathology andInternal Medicine, Vanderbilt University
Medical Center, Nashville, Tennessee, USA; andCenter for Transgene
Technology and Gene Therapy, Flanders Interuniversity Institute for
Biotechnology, University of Leuven, Leuven, Belgium.

There are growing data to suggest that tissue hypoxia represents a
critical force that drives adult vasculogenesis. Vascular endothelial
growth factor (VEGF) expression is dramatically up-regulated by hypoxia
and results in enhanced neovascularization. Although the role of VEGF
in angiogenesis has been well characterized, its role in adult
vasculogenesis remains poorly understood. We used two distinct murine
bone marrow transplantation (BMT) models to demonstrate that increased
VEGF levels at the site of tumor growth promoted vasculogenesis in
vivo.

This effect of VEGF was downstream of its effect to enhance either
mobilization or survival of circulating endothelial progenitor cells
(EPCs). Both VEGFR1 (flt1) and VEGFR2 (flk1) are expressed on culture
expanded human EPCs. Previous studies suggest that the effect of VEGF
on endothelial cell migration is primarily mediated via VEGFR2;
however, VEGF-induced EPC migration in vitro was mediated by both
receptors, suggesting that VEGF-VEGFR1 interactions in EPCs are
distinct from differentiated endothelial cells.

We used specific blocking antibodies to these receptors to demonstrate
that VEGFR1 plays an important role in human EPC recruitment to tumors.
These findings were further supported by our finding that
tumor-associated placental growth factor (PlGF), a VEGFR1-specific
agonist, increased tumor vasculogenesis in a murine BMT model. We
further showed that both VEGF receptors were necessary for the
formation of functional vessels derived from exogenously administered
human ex vivo expanded EPCs.

Our data suggest local VEGF and/or PlGF expression promote
vasculogenesis; VEGF plays a role in EPC recruitment and subsequent
formation of functional vessels.--Li, B., Sharpe, E. E., Maupin, A.,
Teleron, A. A., Pyle, A., Carmeliet, P., Young, P. P. VEGF and PlGF
promote adult vasculogenesis by enhancing EPC recruitment and vessel
formation at the site of tumor neovascularization.

PMID: 16754748 [PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/entrez/quer ... s=16754748

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